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mitomycin c treated mefs  (ATCC)
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ATCC mitomycin c treated mefs
Representative images of mESCs and EBs (A) mESCs maintained on mitomycin C-treated <t>MEFs</t> in ES-DMEM medium. The colonies exhibit compact and rounded morphology, indicative of pluripotency. Scale bar: 200 μm. (B) mESCs cultured on 0.1% gelatin-coated cell culture plate in ES-IMDM medium without MEFs. mESCs display slightly flattened colony morphology compared to feeder-containing culture condition. Scale bar: 200 μm. (C) EBs on 4 days of differentiation in serum differentiation medium. EBs show typical spherical morphology with defined borders. Scale bar: 200 μm.
Mitomycin C Treated Mefs, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mitomycin+c-treated+mefs/pmc12491523-287-16-20?v=ATCC
Average 93 stars, based on 1 article reviews
mitomycin c treated mefs - by Bioz Stars, 2026-07
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mitomycin c treated mef feeders  (Thermo Fisher)
96
Thermo Fisher mitomycin c treated mef feeders
Representative images of mESCs and EBs (A) mESCs maintained on mitomycin C-treated <t>MEFs</t> in ES-DMEM medium. The colonies exhibit compact and rounded morphology, indicative of pluripotency. Scale bar: 200 μm. (B) mESCs cultured on 0.1% gelatin-coated cell culture plate in ES-IMDM medium without MEFs. mESCs display slightly flattened colony morphology compared to feeder-containing culture condition. Scale bar: 200 μm. (C) EBs on 4 days of differentiation in serum differentiation medium. EBs show typical spherical morphology with defined borders. Scale bar: 200 μm.
Mitomycin C Treated Mef Feeders, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mitomycin+c-treated+mefs/pmc13213254-28-14-26?v=Thermo+Fisher
Average 96 stars, based on 1 article reviews
mitomycin c treated mef feeders - by Bioz Stars, 2026-07
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mitomycin c treated mouse embryonic fibroblasts mefs  (Thermo Fisher)
96
Thermo Fisher mitomycin c treated mouse embryonic fibroblasts mefs
Representative images of mESCs and EBs (A) mESCs maintained on mitomycin C-treated <t>MEFs</t> in ES-DMEM medium. The colonies exhibit compact and rounded morphology, indicative of pluripotency. Scale bar: 200 μm. (B) mESCs cultured on 0.1% gelatin-coated cell culture plate in ES-IMDM medium without MEFs. mESCs display slightly flattened colony morphology compared to feeder-containing culture condition. Scale bar: 200 μm. (C) EBs on 4 days of differentiation in serum differentiation medium. EBs show typical spherical morphology with defined borders. Scale bar: 200 μm.
Mitomycin C Treated Mouse Embryonic Fibroblasts Mefs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mitomycin+c-treated+mefs/pm42000973-104-40-56?v=Thermo+Fisher
Average 96 stars, based on 1 article reviews
mitomycin c treated mouse embryonic fibroblasts mefs - by Bioz Stars, 2026-07
96/100 stars
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mitomycin c treated mouse embryonic fibroblast mef feeder layer  (Thermo Fisher)
96
Thermo Fisher mitomycin c treated mouse embryonic fibroblast mef feeder layer
Representative images of mESCs and EBs (A) mESCs maintained on mitomycin C-treated <t>MEFs</t> in ES-DMEM medium. The colonies exhibit compact and rounded morphology, indicative of pluripotency. Scale bar: 200 μm. (B) mESCs cultured on 0.1% gelatin-coated cell culture plate in ES-IMDM medium without MEFs. mESCs display slightly flattened colony morphology compared to feeder-containing culture condition. Scale bar: 200 μm. (C) EBs on 4 days of differentiation in serum differentiation medium. EBs show typical spherical morphology with defined borders. Scale bar: 200 μm.
Mitomycin C Treated Mouse Embryonic Fibroblast Mef Feeder Layer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mitomycin+c-treated+mefs/pm41922802-64-4-23?v=Thermo+Fisher
Average 96 stars, based on 1 article reviews
mitomycin c treated mouse embryonic fibroblast mef feeder layer - by Bioz Stars, 2026-07
96/100 stars
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cf 1 mitomycin c treated mef cells  (Thermo Fisher)
96
Thermo Fisher cf 1 mitomycin c treated mef cells
Representative images of mESCs and EBs (A) mESCs maintained on mitomycin C-treated <t>MEFs</t> in ES-DMEM medium. The colonies exhibit compact and rounded morphology, indicative of pluripotency. Scale bar: 200 μm. (B) mESCs cultured on 0.1% gelatin-coated cell culture plate in ES-IMDM medium without MEFs. mESCs display slightly flattened colony morphology compared to feeder-containing culture condition. Scale bar: 200 μm. (C) EBs on 4 days of differentiation in serum differentiation medium. EBs show typical spherical morphology with defined borders. Scale bar: 200 μm.
Cf 1 Mitomycin C Treated Mef Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mitomycin+c-treated+mefs/pmc12963348-194-4-21?v=Thermo+Fisher
Average 96 stars, based on 1 article reviews
cf 1 mitomycin c treated mef cells - by Bioz Stars, 2026-07
96/100 stars
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mitomycin c treated mefs  (Selleck Chemicals)
96
Selleck Chemicals mitomycin c treated mefs
Representative images of mESCs and EBs (A) mESCs maintained on mitomycin C-treated <t>MEFs</t> in ES-DMEM medium. The colonies exhibit compact and rounded morphology, indicative of pluripotency. Scale bar: 200 μm. (B) mESCs cultured on 0.1% gelatin-coated cell culture plate in ES-IMDM medium without MEFs. mESCs display slightly flattened colony morphology compared to feeder-containing culture condition. Scale bar: 200 μm. (C) EBs on 4 days of differentiation in serum differentiation medium. EBs show typical spherical morphology with defined borders. Scale bar: 200 μm.
Mitomycin C Treated Mefs, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mitomycin+c-treated+mefs/bio_rxiv__64898__2025__12__30__686824-233-22-33?v=Selleck+Chemicals
Average 96 stars, based on 1 article reviews
mitomycin c treated mefs - by Bioz Stars, 2026-07
96/100 stars
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mitomycin c treated mouse embryonic fibroblast cells mefs  (Fisher Scientific)
86
Fisher Scientific mitomycin c treated mouse embryonic fibroblast cells mefs
Representative images of mESCs and EBs (A) mESCs maintained on mitomycin C-treated <t>MEFs</t> in ES-DMEM medium. The colonies exhibit compact and rounded morphology, indicative of pluripotency. Scale bar: 200 μm. (B) mESCs cultured on 0.1% gelatin-coated cell culture plate in ES-IMDM medium without MEFs. mESCs display slightly flattened colony morphology compared to feeder-containing culture condition. Scale bar: 200 μm. (C) EBs on 4 days of differentiation in serum differentiation medium. EBs show typical spherical morphology with defined borders. Scale bar: 200 μm.
Mitomycin C Treated Mouse Embryonic Fibroblast Cells Mefs, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mitomycin+c-treated+mefs/pm41354677-61-8-15?v=Fisher+Scientific
Average 86 stars, based on 1 article reviews
mitomycin c treated mouse embryonic fibroblast cells mefs - by Bioz Stars, 2026-07
86/100 stars
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Representative images of mESCs and EBs (A) mESCs maintained on mitomycin C-treated MEFs in ES-DMEM medium. The colonies exhibit compact and rounded morphology, indicative of pluripotency. Scale bar: 200 μm. (B) mESCs cultured on 0.1% gelatin-coated cell culture plate in ES-IMDM medium without MEFs. mESCs display slightly flattened colony morphology compared to feeder-containing culture condition. Scale bar: 200 μm. (C) EBs on 4 days of differentiation in serum differentiation medium. EBs show typical spherical morphology with defined borders. Scale bar: 200 μm.

Journal: STAR Protocols

Article Title: Protocol for chromatin immunoprecipitation and hematovascular function assays with differentiated mouse embryonic stem cells

doi: 10.1016/j.xpro.2025.104083

Figure Lengend Snippet: Representative images of mESCs and EBs (A) mESCs maintained on mitomycin C-treated MEFs in ES-DMEM medium. The colonies exhibit compact and rounded morphology, indicative of pluripotency. Scale bar: 200 μm. (B) mESCs cultured on 0.1% gelatin-coated cell culture plate in ES-IMDM medium without MEFs. mESCs display slightly flattened colony morphology compared to feeder-containing culture condition. Scale bar: 200 μm. (C) EBs on 4 days of differentiation in serum differentiation medium. EBs show typical spherical morphology with defined borders. Scale bar: 200 μm.

Article Snippet: Alternatives: For MEFs, alternatives to EmbryoMax Primary MEFs (Passage 3) include commercially available gamma irradiated or mitomycin C-treated MEFs from ATCC (SCRC-1040), GlobalStem/Thermo Fisher (GSC-6001G), Applied StemCell (ASF-1101).

Techniques: Cell Culture